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13. Treatment for High-Risk Nonbleeding Ulcers - Journal Watch (General)
- general-medicine.jwatch.org
- , ulcers with visible vessels or adherent clots). ...
- The effect of endoscopic therapy in patients receiving omeprazole for bleeding ulcers with nonbleeding visible vessels or adherent clots: A randomized comparison. ...
14. A Prestressed Cable Network Model of the Adherent Cell Cytoskeleton -- Coughlin and Stamenovic 84 (2): 1328 -- Biophysical Journal
- www.biophysj.org
- A Prestressed Cable Network Model of the Adherent Cell Cytoskeleton .
- A prestressed cable network is used to model the deformability of the adherent cell actin cytoskeleton. ... The models were deformed to mimic cell poking (CP), magnetic twisting cytometry (MTC) and magnetic bead microrheometry (MBM) measurements on living adherent cells. ...
- Many biological functions of adherent cells are partly regulated through cell shape and deformability (Folkman and Mascona, 1978; Elson, 1988; Singhvi et al. ... A growing body of evidence indicates that the primary control of adherent cell shape and deformability is exerted at the level of the cytoskeletal (CSK) filaments. ...
- We recently used a conceptual cable network model to connect microstructrual CSK parameters to macroscopic properties of adherent cells (Stamenovi and Coughlin, 1999). ...
- In the current study, two prestressed cable networks were examined as models of the adherent cell actin CSK. ... The models were deformed to mimic cell poking (CP), magnetic twisting cytometry (MTC), and magnetic bead microrheometry (MBM) measurements on living adherent cells. ...
- The adherent cell CSK was modeled as a network of elastic cables interconnected by frictionless pin-joints. ...
- FIGURE 1 (A) Hexagonal and (B) triangluar cable network models for the adherent cell cytoskeleton. ...
- Because there is no direct observation of l0 in living cells, l0 was estimated from an effective CSK pore size (Dp) in adherent cells. Experimental observations of the actin CSK in adherent endothelial and fibroblast cells reveal a network with Dp 50200 nm (Luby-Phelps et al. ...
- The models were deformed to mimic CP, MTC, and MBM measurements on adherent cells. ...
- The CP measurement technique has been used to assess the mechanical properties of various adherent cells (McConnaughey and Petersen, 1980; Petersen et al. ...
- The indentation of the stylus (u) on adherent cells was mimicked in the model by imposing the following constraints on the positions of the pin-joints: (1) the vertical Z-coordinates of the pin-joints under the punch were such that z -u and (2) the X- and Y-coordinates of the pin-joints located outside the punch diameter were not allowed to penetrate the side of the punch, x2 + y2 > (D/2)2 when z > -u. ...
- The relationship between u and W was compared to data obtained from CP measurements during the indentation of adherent fibroblast cells. ...
15. Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells -- Wang et al. 282 (3): C606 -- AJP - Cell Physiology
- ajpcell.physiology.org
- Stiffness and prestress are closely associated in adherent contractile cells .
- Here we have tested that prediction in adherent human airway smooth muscle (HASM) cells. ...
- Mechanical anisotropy of adherent cells probed by a three-dimensional magnetic twisting device.
- Cytoskeletal mechanics in adherent human airway smooth muscle cells: probe specificity and scaling of protein-protein dynamics.
16. Expression and regulation of chemokine receptors in human natural killer cells -- Inngjerdingen et al. 97 (2): 367 -- Blood
- www.bloodjournal.org
- 2 These cells are generated by adherence to plastic flasks after activation with interleukin (IL)-2 and are designated as adherent lymphokine-activated killer (AD-LAK) cells. ... 6 In a more related work, Vujanovic et al7 showed that AD cells migrate significantly better than NA cells into the spheroids of human squamous cell carcinoma of the head and neck or breast carcinoma, emphasizing the antitumor therapeutic potential of adherent IL-2-activated NK cells. ... 6-9 However, the mechanisms contributing to the elimination of tumors, and to the close contact between adherent cells and tumor cells, are to a large extent not known. ...
- Here, we systematically investigated the expression of chemokine receptors in nonactivated NK cells and compared this expression with the expression of these receptors in IL-2-activated (adherent "AD" versus nonadherent "NA") cells. ...
- 22 Twenty-four hours later, 2 populations were separated, those that are plastic adherent (AD cells) and those that are nonadherent (NA cells). ...
- Expression of CXC and CX3C chemokine receptors on the surface of nonactivated, adherent, and nonadherent cells (NK, AD, and NA, respectively). ...
- Lanes 2, 3, and 4 show mRNA of protected probes in nonactivated (NK), adherent (AD), and nonadherent (NA) cells. ...
- Expression of CC chemokine receptors on the surface of nonactivated (NK), adherent (AD), nonadherent (NA), and T cells. ...
- Expression of mRNA in nonactivated (NK), adherent (AD), and nonadherent (NA) cells using the hCR5 multitemplate kit, which detects mRNA for CCR1, CCR2, CCR3, CCR4, CCR5, and CCR8. ...
- Expression of mRNA in nonactivated (NK), adherent (AD), and nonadherent (NA) cells was determined for various chemokines using the hCK5 multitemplate kit. ...
- Here, we examined the expression of chemokine receptors in nonactivated NK cells, as well as in IL-2 activated NK cells that either adhere to plastic flasks (adherent, AD) or those that do not adhere (nonadherent, NA). ...
- A new approach to generating antitumor effectors for adoptive immunotherapy using human adherent lymphokine-activated killer cells. ... Accumulation of adoptively transferred adherent, lymphokine-activated killer cells in murine metastases. ...
17. Mechanical properties of cultured human airway smooth muscle cells from 0.05 to 0.4 Hz -- Maksym et al. 89 (4): 1619 -- Journal of Applied Physiology
- www.jap.org
- Cytoskeletal mechanics in adherent human airway smooth muscle cells: probe specificity and scaling of protein-protein dynamics.
- A Prestressed Cable Network Model of the Adherent Cell Cytoskeleton.
- Stiffness and prestress are closely associated in adherent contractile cells.
18. Activation of Human Neutrophil by Cytokine-Activated Endothelial Cells -- Takahashi et al. 88 (4): 422 -- Circulation Research
- www.circresaha.org
- Neutrophils Adherent to Cytokine-Activated ECs.
- The number of neutrophils adherent to ECs was counted using a phase-contrast microscope (Nikon Diaphot). ...
- Neutrophils adherent to activated ECs and ICAM-1 expression on IL-1activated or TNF-activated ECs. We counted the number of neutrophils adherent to activated ECs with a phase-contrast microscopy after 5 minutes of coculture (A). ...
- Adenosine inhibits priming of neutrophils and the respiratory burst of cytokine-triggered adherent neutrophils via A2 receptor. ...
- On the basis of these findings, we speculate that, first, neutrophil recruitment via mediators and cytokines produced by activated ECs, such as PAF and IL-8, is an initial step; second, activated EC-associated PAF primes neutrophils adherent to ECs via the Mac-1ICAM-1 pathway; and, third, potentially more GM-CSF synthesized by activated ECs augmented neutrophil activation during the transendothelial migration. ...
- Adenosine regulates the respiratory burst of cytokine-triggered human neutrophils adherent to biologic surface. ...
19. Opposing views on tensegrity as a structural framework for understanding cell mechanics -- Ingber et al. 89 (4): 1663 -- Journal of Applied Physiology
- www.jap.org
- Furthermore, every time I breath in, causing the muscles of my neck and chest to pull out on my lattice of ribs, my lung expands, alveoli open, taught bands of elastin in the extracellular matrix (ECM) relax, buckled bundles of cross-linked (stiffened) collagen filaments straighten, basement membranes tighten, and the adherent cells and cytoskeletal filaments feel the pull; however, nothing breaks and the deformation is reversible. ...
- Phase-contrast (left) and polarization optic (right) views of an adherent endothelial cell immediately before (top) and after (bottom) a fibronectin-coated micropipette (visible in bottom) was bound to integrin receptors on its surface and pulled laterally (downward in this view) using a micromanipulator. ...
- This has been demonstrated directly in studies in which cytoskeletal prestress was altered by modulating actomyosin-based contractility using drugs (19), transfecting cells with constitutively active myosin light chain (MLC) kinase (3), varying transmembrane osmotic forces (3) or quickly distending the flexible ECM substrate on which the cell is adherent (34), resulting in immediate changes in the cellular shear modulus (a quantitative measure of stiffness or shape stability). ...
- Colchicine was used to disrupt microtubules in adherent cells that were activated with a saturating dose of the chemical constrictor histamine, again to ensure optimal MLC phosphorylation. ...
- Local measurements of viscoelastic parameters of adherent cell surfaces by magnetic bead microrheometry. ...
- Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells? Am J Physiol Cell Physiol 274: C1283-C1289, 1998 Abstract/Free Full Text .
- The role of prestress and architecture of the cytoskeleton and deformability of cytoskeletal filaments of adherent cells: a quantitative approach. ...
- Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells? Am J Physiol Cell Physiol 274: C1283-C1289, 1998 Abstract/Free Full Text .
- Is cytoskeletal tension a major determinant of cell deformability in adherent endothelial cells? Am J Physiol Cell Physiol 274: C1283-C1289, 1998 Abstract/Free Full Text .
- Stiffness and prestress are closely associated in adherent contractile cells.
20. 9/18/97 - Hubbard Adherent's School Bid on Hold
- www.factnet.org
- 9/18/97 - Hubbard Adherent's School Bid on Hold .
- Hubbard Adherent's School Bid on Hold .
21. A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation -- Ito et al. 19 (4): 562 -- The EMBO Journal
- embojournal.npgjournals.com
- (A) Cells were lysed at 30 min after non-adherent culture (suspension), and 30 (FN 30min) and 90 (FN 90min) min after plating on FN. ...
- Cells were lysed 30 min after non-adherent cell cultivation and immunoprecipitated with PP2A C subunit Ab. ...
- Melanoma cells were cultured under non-adherent conditions for 1 h, lysed and blotted (left column). ...
- Bacterial grade dishes (Becton Dickinson) were used for non-adherent culture conditions; adherent culture conditions were produced by coating the dishes with FN (10 µg/ml; Gibco-BRL). ...
- (1996) Integrin v5-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin. ...
22. Macrophages as Effector Cells in Interleukin 12-induced T Cell-dependent Tumor Rejection -- Tsung et al. 62 (17): 5069 -- Cancer Research
- cancerres.aacrjournals.org
- This fixation was found to be sufficient to kill all cells, because no viable adherent cells were seen after overnight culture in medium. ... The number of adherent cells was determined by CellTiter 96 Aqueous One Cell proliferation kit (Promega Corp. ...
- Under such conditions, macrophages in the coculture did not become adherent, and the efficiency of tumor cell killing was indicated by the number of adherent tumor cells 2 days after the start of the coculture. ...
- Antitumor effect of Lyt-1+2-T cells depends on the existence of adherent cells. ...
23. Diarrheagenic Escherichia coli -- Nataro and Kaper 11 (1): 142 -- Clinical Microbiology Reviews
- cmr.asm.org
- DIFFUSELY ADHERENT E. ...
- (139) and remains the "gold standard" for the diagnosis of EAEC and diffusely adherent E. ... However, collaborative studies have shown that the assay performed essentially as first described provides the best ability to differentiate among all three adherent diarrheagenic categories (EPEC, EAEC, and DAEC) (678). ...
- Marked cytoskeletal changes, including accumulation of polymerized actin, are seen directly beneath the adherent bacteria; the bacteria sometimes sit upon a pedestal-like structure. ...
- In this test, fluorescein isothiocyanate (FITC)-labeled phalloidin binds specifically to filamentous actin in cultured epithelial cells directly beneath the adherent bacteria. ...
- The tyrosine-phosphorylated proteins are part of the A/E lesion, and the distribution of the phosphorylated proteins is restricted to an area immediately beneath the adherent bacteria at the tip of the pedestals (545). ...
- Proximal small intestinal mucosal biopsy specimens often, but not always, show intimately adherent bacteria and the classic A/E histopathology (194, 547, 592). ...
- (303) used a monoclonal antibody directed against -actinin, which is also concentrated in epithelial cells directly beneath the adherent EPEC. ...
24. Involvement of Apoptosis and Interferon-{gamma} in Murine Toxoplasmosis -- Shen et al. 42 (9): 2031 -- Investigative Ophthalmology & Visual Science
- www.iovs.org
- Sher, A, Oswald, IP, Hieny, S, Gazzinelli, RT (1993) Toxoplasma gondii induces a T-independent IFN-gamma response in natural killer cells that requires both adherent accessory cells and tumor necrosis factor-alpha J Immunol 150,3982-3989 Abstract/Free Full Text .
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